Journal: iScience

Article Title: Aurkb deficiency disrupts microglial development, homeostasis and hinders remyelination following cuprizone-induced demyelination

doi: 10.1016/j.isci.2026.114718

Figure Lengend Snippet: Aurkb deficiency in microglia disrupts remyelination and oligodendrocyte density in the CPZ-induced demyelination model (A) Adult Aurkb fl/fl and Cx3cr1 CreERT2/+ Aurkb fl/fl mice (2–3 months old) were i.p. injected with TAM for 5 consecutive days. After one month, the mice were fed a 0.25% CPZ-enriched diet for 5 weeks to induce demyelination (CPZ group) or followed by a CPZ-free normal diet for an additional 2 weeks (Recovery group). The corpus callosum (CC) was analyzed by immunofluorescence (IF) and Black-Gold II myelin staining. (B and C) Representative Black-Gold II myelin staining images and quantification of myelin intensity ( n = 5 mice per genotype per group, Scale bars: 200 μm). (D–F) Representative immunofluorescence analysis and quantification of total Mbp and oligodendrocytes (Olig2 + ), and g-h) representative immunofluorescence analysis and quantification of mature oligodendrocytes (CC-1 + ) ( n = 5 mice per genotype per group, Scale bars: 50 μm). Data are presented as the mean ± SD. two-way ANOVA with Bonferroni multiple comparisons test in (C, E, F, and H); ∗∗ p < 0.01 and ∗∗∗ p < 0.001; ns, not significant compared with the Aurkb fl/fl group.

Article Snippet: The following primary antibodies were used: rabbit anti-Iba-1 antibody (1:500, Wako, Cat: 019–19741), mouse anti-Iba-1 antibody (1:400, Abcam, Cat: ab283319), rat anti-CD206 antibody (1:200, Bio-Rad, Cat: MCA2235), rat anti-BrdU antibody (1:400, Abcam, Cat: ab6326), mouse anti-phospho-Histone H3 (Ser10) antibody (1:200, Cell Signaling Technology, Cat: 9706S), mouse anti-APC (1:100, CC-1, Merck, Cat: OP80), rat anti-myelin basic protein (Mbp) monoclonal antibody (1:500, Abcam, Cat: ab7349), rabbit anti-degraded myelin basic protein (dMbp) antibody (1:2000, Millipore Sigma, Cat: AB5864), rabbit anti-Olig2 antibody (1:500, Proteintech, Cat: 13999-1-AP) and rat anti-CD68 antibody (1:500, Abcam, Cat: ab53444).

Techniques: Injection, Immunofluorescence, Staining